RESUMEN DE ARTÍCULO CITADO:
In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes
Guerreiro Tanomaru, Juliane Maria, et al. Fernanda Geraldes Pappen, Mário Tanomaru Filho, Denise Madalena Palomari Spolidorio, Izabel Yoko Ito.
Abstract
The aim of this study was to evaluate the antimicrobial activity of different trademarks and compositions of gutta-percha points and calcium hydroxide pastes used in endodontic therapy. The evaluated material consisted of gutta-percha points containing calcium hydroxide (RoekoTM), gutta-percha points containing chlorhexidine (RoekoTM), two convencional gutta-percha points (Endo PointsTM and RoekoTM) and two calcium hydroxide pastes (CalenTM and Calen/PMCCTM). Antimicrobial tests included five species of microorganisms: Escherichia coli (ATCC10538), Staphylococcus epidermidis (ATCC12228), Staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC27853), and Micrococcus luteus (ATCC9341). The Agar difusion method was employed.
The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37°C for 24 h. The triphenyltetrazolium chloride gel was added for optimization and the zones of inhibition were measured. Statistical evaluation was carried out using analysis of variance and Tukey Test. The obtained results showed that all microbial species used in the study were inhibited by the gutta-percha points containing chlorhexidine and by the calcium hydroxide pastes (CalenTM and Calen/PMCCTM), with similar results (p > 0.05). No antimicrobial activity was observed for the other groups. It was concluded that the gutta-percha points containing chlorhexidine presented antimicrobial activity, whereas the gutta-percha points containing calcium hydroxide did not.
Guerreiro Tanomaru, Juliane Maria, et al. In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes. Braz Oral Res 2007;21(1):35-9 (Abstract)
Investigado por Claudia Patricia Cárdenas Cuellar, Alumna del grupo 1501-2009, FES Iztacala, UNAM
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